The Shared Characteristics Of Spheroids And Tumors
Since its introduction, 3D cell culture has revolutionized cancer research by providing an efficient and modifiable research platform, that recapitulates in vivo like cell structure, function, gene expression and tissue microenvironment, all of which are important parameters for the drug
Organoid tumor models: benefits and challenges
Cancer is one of the most threatening diseases and leading cause of death worldwide (1). Intra- and intertumoral heterogeneity is often responsible for metastasis formation, treatment failure, and therapy resistance. The increase in the cost of research and development of
Types of three-dimensional cultures for drug screening
The rather low success rates in new drug development and the increased costs suggest that alternative and robust strategies are required to improve early drug discovery. Two-dimensional (2D) monolayer cultures do not provide a true in vitro representation of disease and
Using Biopolymers And Micromachining Technology In Generating Multicellular Spheroids
Multicellular spheroids (MCSs) are formed through spontaneous aggregation of cells in the presence of adhesion molecules known as cadherins (1). Decades of research have proved MCSs to be an efficient 3D cell culture model for a wide array of research.
The most common primary cells used in research
Human primary cells are isolated directly from human tissues and maintain the physiological characteristics of their original tissue. This article discusses the most common primary cells used by scientists (1,2). Endothelial cells In the human body, endothelial cells form a single-cell thick-walled
Key differences between cancer cells and normal cells
Normally, human cells grow and divide to form new cells and as the cells grow old or are damaged, they are replace with new cells. When this order of cell cycle breaks down and cells proliferate abnormally, they form tumors
Why three-dimensional cell cultures are popular among cancer researchers
Cancer disease being the second-leading cause of death worldwide is a highly studied subject (1). The models used by cancer researchers are continuously evolving and advancing to better understand cancer invasion, progression, and early detection. Most cancer studies have been
How to create three-dimensional cell culture models
A three-dimensional (3D) cell culture is an artificially created environment in which cells grow or interact with their surroundings in all three dimensions. This cell culture is becoming increasingly popular to replace the traditional two-dimensional (2D) monolayer because it correctly
Evolution Of 3D Cell Culture
In the past decade research has experienced drastic changes in the development of an alternative research method over the traditional use of animal models as an investigative platform. 2D Monolayer in vitro cultures are effective as drug screening platform but
Is It The Right Time to Advance from 2D – 3D Cell Culture
Since the early 1900s, the two-dimensional (2D) cell culture method is used to culture cells and grow them in favorable artificial conditions (1) thereby playing a vital role in biomedical research (2). In line with the limitations of 2D cell