A spheroid is a three-dimensional (3D) cell culture model that mimics the in vivo environment more closely than traditional two-dimensional (2D) monolayer cultures. Physiologically, spheroids exhibit several distinct characteristics compared to 2D cell cultures. This…
As spheroids grow, oxygen diffusion from the external layers of cells into the core gets limited and this leads to cellular stress, energy deprivation, and eventually necrosis. The development of necrosis in spheroids depends on…
Spheroid cell cultures are defined as 3-dimentional cell aggregates that are formed via spontaneous self-assembly of cells in a cell repellant, non-adhesive surface. It is by far one of the popular anchorage free 3D cell…
The term necrosis refers to cell or tissue death and is typically caused by factors such as injury, infection, or inadequate blood supply. Necrosis can also form within a spheroid, which is a 3D culture…
Spheroids are multicellular cell aggregates that self-assemble in 3D structures when cultivated in an environment that prevents attachment to the artificial surface, such as BIOFLOAT 96-well plates with their anti-adhesive properties. Spheroids can be generated…
Tumor spheroids are three-dimensional cell culture models that have gained significant attention in cancer research because they provide a more physiologically relevant environment for studying tumor biology compared to traditional two-dimensional cell culture systems. 3D…
Spheroid are defined as 3-dimentional cell aggregates that are formed via spontaneous self-assembly of cells when cultivated on a cell repellant, non-adhesive surface. Spheroids are by far one of the most popular scaffold-free 3D cell…
Spheroids grow in three dimensions to form a sphere-like structure which mimics cell in vivo microenvironment more closely than conventional 2D monolayer cell culture. They can be formed from single or multiple cell types that…
Multicellular spheroids (MCSs) are formed through spontaneous aggregation of cells in the presence of adhesion molecules known as cadherins (1). Decades of research have proved MCSs to be an efficient 3D cell culture model for…
Since the introduction of monolayer cell cultures, cell culture methods have advanced exponentially in the past decade, moving from traditional 2D cell culture methods to 3D cultures. While 2D cultures are simple and allows for…