In the recent decades, 3D bioprinting methods have been widely used in tissue engineering and regenerative medicine. Additionally, these techniques have also emerged as a novel approach in the development of in vivo-like 3D cell…
A spheroid culture system provides a similar physiochemical environment to in vivo, by facilitating cell-cell and cell-matrix interaction to overcome the limitations of traditional monolayer cell cultures. Due to the importance of stem cell culture…
Solid tumors are composed of a number of different types of cells. These include cancer cells, stromal cells such as fibroblasts, immune cells, lymphatic endothelial cells, vascular endothelial cells, pericytes and adipocytes (1). These cells…
Spheroids are recognized as physiologically relevant three-dimensional models that could capture the major characteristics of both healthy and diseased tissues. Considering its high cell density, increased deposition of extracellular matrix and accelerated proliferation rates, spheroid-based…
Spheroids, which are self, assembled cellular aggregates, as a representative 3D model of solid tumors is largely dependent on the uniformity of its size and shape. Uniform spheroids yield reproducible results in drug screens as…
Spheroid model has been shown to have features consistent with patient specific Non-Small Cell Lung Cancer Spheroid tumours (NSCLC), characterised by extremely high clonal and morphological heterogeneity of tumours and multi-drug resistance. Lung malignancies accounts…
To replicate in vitro conditions of extra cellular matrix proteins presents within a tumour, Matrigel is used as both a matrix and additive to aid in spheroid formation. While some cell lines form dense macromolecular…
Modern drug discovery processes has encountered significant setbacks in the past decades despite the technological advances made in analytical tools and research model development. This is primarily because of the use of inadequate biological models…
Spheroid models used in drug screens offer the benefit of a physiologically relevant biological model to allow for screening of cancer killing therapeutics or drugs toxicity to host cells. Spheroid culture systems are easy to…
Cancer stem cells constitute a fraction of cancer cells, that are able to self renew, and differentiate to generate entire tumor structures. These cells were discovered in the 1990s, in hemopoietic cancers, and subsequently reported…