Spheroids Embedded in Matrigel
To replicate in vitro conditions of extra cellular matrix proteins presents within a tumour, Matrigel is used as both a matrix and additive to aid in spheroid formation. While some cell lines form dense macromolecular structures, others form loose aggregates
3D Spheroid Technology Use in Drug Screening
Modern drug discovery processes has encountered significant setbacks in the past decades despite the technological advances made in analytical tools and research model development. This is primarily because of the use of inadequate biological models that do not mimic in-
Analysing Drug Response in 3D Spheroid Culture Models
Spheroid models used in drug screens offer the benefit of a physiologically relevant biological model to allow for screening of cancer killing therapeutics or drugs toxicity to host cells. Spheroid culture systems are easy to use, modifiable, adaptable to standards
Cultivating spheroids from primary cancers and cancer stem cells
Cancer stem cells constitute a fraction of cancer cells, that are able to self renew, and differentiate to generate entire tumor structures. These cells were discovered in the 1990s, in hemopoietic cancers, and subsequently reported to be present in solid
Stem cell spheroids – A vital tool in regenerative medicine
Stem cells serve as the primary resource in regenerative medicine including tissue engineering and transplantation therapy. To this effect human mesenchymal stem cells contain secretory factors with anti-inflammatory, angiogenic and immune regulation factors that makes them an efficient resource for
Generating large scale primary liver spheroids
Liver spheroids are successful as a physiologically relevant 3D cell culture model, with accurate representation of in-vivo like structure and function. In addition these 3D platforms are compatible with most high throughput, and high content screening systems, thus proving to
SPHEROID BASED APPROACHES FOR DRUG SCREENING
Over the past several years, various types of 3D cultures systems have acquired attention and increased recognition as an important tool for research. This trend has also been illustrated, In Alison Abbotts 2003 nature report titled: ´CELL CULTURE – BIOLOGY´S
Perfect 3D spheroids for cancer research with BIOFLOAT
Cancer research relies on cell based model systems to understand the growth and progression of tumors, and subsequently the discovery of new therapeutic drugs. Traditional monolayer cultures have been widely used in this regard due to its ability to generate