PERFECT 3D SPHEROIDS WITH BIOLFOAT

Cancer research relies on cell based model systems to understand the growth and progression of tumors

Cancer research relies on cell based model systems to understand the growth and progression of tumors, and subsequently the discovery of new therapeutic drugs. Traditional monolayer cultures have been widely used in this regard due to its ability to generate well-controlled homogenous cell environment and ease of microscope analysis. However 2D cultures are unable to recapitulate the in-vivo microenvironment of the cells and as a result most drug screenings done on 2D monolayer cells falls short when transferred to animal models or humans.

Spheroids are commonly cultured in well plates coated with special coating such as BIOFLOAT Flex coating solution. These coating ensures a hydrophilic cell repellant coating forcing the cells to self-aggregate. Spheroid systems can be modified by; i) spheroid morphology, ii) spheroid number, iii) spheroid formation over time, iv) spheroid size, and v) cell line and co-culture.

To this end, BIOFLOAT Flex or Pre coated plates offer the following advantages :

When compared with benchmark plates, BIOFLOAT coated plates allow the formation of one round cell spheroids per well, which is an important criterion for generating a reproducible, reliable cell and high-throughput analysis.
In most cell culture systems the size of spheroid is determined by the initial seeding density, which can differ between cell lines.
To recapitulate the cellular heterogeneity found in solid tumors, spheroids are often cultured in co-cultures of different ratios of cancer to stromal cells to better mimic the in vivo conditions Most often, one cell line acts as feeder cells for the other cell line.

For cancer research it is of importance to generate a modifiable in vitro platform to understand the growth and progression of solid tumors. In this regard, BIOFLOAT spheroid culture system is an excellent choice that allows generation of round and uniform spheroids, in which spheroid morphology can be fine-tuned easily by modifying initial seeding number or cell line model.

Here you will see that we’ve offered a extensive online resources from faCellitate experts, who have shared their knowledge, studies, advice, and much more to help you off to a fantastic start. Check out how you can use faCellitate polymer solutions to customize your cell culture.

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