Cell Culture Insights

Go 3D with Biofloat : 3D cell culture

3D CELL CULTURE 

BIOFLOAT™ coatings from faCellitate provide a highly defined, fully inert cell- and protein-repellent surface.

The polymeric coating enables a fast er generation of 3D spheroids, which shortens your experimental timeline and prevents the formation of satellites and irregular aggregates.

Choose between the ready-to-use 96-well plate or DIY BIOFLOAT FLEX coating solution to modify a broad range of plastic and glass surfaces. One pipetting or rinsing step is sufficient to passivate your surface on the nanometer scale.

Create a homogenous and robust coating for your cell culture vessels, microfluidic devices or chips, which is stable under standard culture conditions.

The consistent quality of the BIOFLOAT surface makes it a premium product for 3D cell culture, ensuring reproducible data for all kinds of cell lines, including cells that fail to generate spheroids on existing products.

CELL CULTURE ANALYSIS 

Cell Culture Analysis

The BioProfile® FLEX2 combines Nova Biomedical’s MicroSensor™ Card technology with optical measurement and freezing point osmometry to provide automated and comprehensive cell culture analysis. The system eliminates chemistry sensor maintenance, increases analyzer speed, and reduces sample volume.

The full 16-test cell culture menu includes glucose (Gluc), lactate (Lac), glutamine (Gln), glutamate (Glu), ammonium (NH₄⁺), sodium (Na⁺), potassium (K⁺), calcium (Ca²⁺), pH, pCO₂, pO₂, total cell density, viable cell density, viability, cell diameter, and osmolality.

Also available is the BioProfile® FLEX2 On-Line Autosampler (OLS), a modular system that connects up to 10 bioreactors to a FLEX2 analyzer for automated sampling and analysis of key cell culture parameters, including pH, gases, metabolites, osmometry, cell density, and cell viability.

The OLS uses small sample volumes and provides fast analysis, allowing automated sampling and analysis of 10 bioreactors in less than 1.5 hours. Setup time for a full 10-bioreactor system requires less than 20 minutes. With proven data comparability to manual sampling methods, the FLEX2 OLS can be connected to OPC-compliant control systems for automated sampling, analysis, and feedback control.