Multicellular spheroids are formed through spontaneous aggregation of cells in the presence of adhesion molecules known as cadherins (1). Since its first implementation in 1952 (2), multicellular spheroids have served as an efficient 3D cell culture model for a wide

Cellular viability plays an important role when determining the efficacy and reliability of a MCTs model. There are many established viability assays such as colorimetric, luminescence and fluorescence assays. However these assays are well suited and optimized for traditional 2D

Despite the much advancement seen in tumor related research and drug discovery, cancer research still experiences a significant amount of challenges due the complex behaviour of tumor cells. Multicellular tumor spheroids (MCTs) bear remarkable similarities to in vivo solid tumors

Multicellular tumor (MCT) spheroids are an essential tool in tumor related research, and drug screens. MCT can be defined as aggregates of heterogeneous cells, closely packed with highly dense spheroids. In order to use MCTs in physiological research and clinical

Multicellular tumor (MCT) spheroids are an essential tool in tumor related research, and drug screens. MCT can be defined as aggregates of heterogeneous cells, closely packed with highly dense spheroids. These cells exhibit strong cell-cell and cell-extracellular matrix (ECM) interactions

Multicellular tumor (MCT) spheroids have become a vital tool in advancing tumor research in pathophysiological front, as well as in developing effective therapeutics. However, its reliability and reproducibility is determined by several factors one of which is the uniformity of

MCTs can be cultivated in scaffold free or scaffold based 3D cultures. Scaffold based 3D cultures includes the use of a 3D artificial matrix or hydrogels as a anchorage for the cells, as well as facilitating the formation of cell-extracellular

Multicellular tumor spheroids are fast becoming an essential tool in tumor related research, and drug screens. MCT can be defined as clusters of heterogeneous cells, formed by self-aggregation, closely packed with highly dense spheroids. These cells exhibit strong cell-cell and

Embedded spheroid models recreate the complex interactions between cells and extracellular matrix and cell types within the contents of secondary cell types and tumour heterogeneity. The combination of natural matrices with spheroids lends itself to study cancer invasion, progression and