Organoids are in vitro miniaturized three-dimensional (3D) model systems of organs that gained huge interest in the last decade. Organoids are self-organizing culture systems generated from stem cells or tissue-specific progenitor cells. Stem cells divide indefinitely and produce different types…
Organoid models are a popular and reliable method in 3D cell culture. Organoids are 3D cellular structures, derived from either stem cells or organ specific progenitor cells. When provided with an external scaffold like Matrigel, they form a complex cluster…
Human primary cells are isolated directly from human tissues and maintain the physiological characteristics of their original tissue. This article discusses the most common primary cells used by scientists (1,2). Endothelial cells In the human body, endothelial cells form a…
Primary cell cultures refer to the cultivation of cells that are directly isolated from human or animal tissues and processed to grow under controlled conditions in vitro. They represent the in vivo state of cells more closely than immortalized cell…
Cell culture is one of the crucial tools in basic scientific research. Cell culture refers to the cultivation in a controlled environment of cells derived from an animal or a plant. Today, various cell culture techniques are used in laboratories…
Cell lines are an easy and inexpensive tool used by researchers routinely. With the plethora of cell lines available today, selecting the right cell line for your project is fundamental for getting reliable results. Cell line contamination, misidentification, source, genetics,…
Normally, human cells grow and divide to form new cells and as the cells grow old or are damaged, they are replace with new cells. When this order of cell cycle breaks down and cells proliferate abnormally, they form tumors…
Cancer disease being the second-leading cause of death worldwide is a highly studied subject (1). The models used by cancer researchers are continuously evolving and advancing to better understand cancer invasion, progression, and early detection. Most cancer studies have been…
A three-dimensional (3D) cell culture is an artificially created environment in which cells grow or interact with their surroundings in all three dimensions. This cell culture is becoming increasingly popular to replace the traditional two-dimensional (2D) monolayer because it correctly…
Stem cells are undifferentiated cells that have the capacity to self-renew and differentiate into specialized cells under given conditions. Stem cells mainly include embryonic stem cells (ESC), induced pluripotent stem cells (iPSC), Epiblast-derived stem cells (Epi-SC), and adult tissue stem…