ORGANOIDS FOR CYSTIC FIBROSIS AND THE LIVING CANCER
Human organoids are a vital source for precision medicine. They can be useful to select an appropriate drug for the patients with genetic diseases or cancer. Cystic fibrosis is a relatively common genetic disease with approximately 90,000 patients suffering with
Mammospheres leading the way in breast cancer research
The advances in spheroid technology in particular, neurospheres has led to establishment of “mammospheres”; a mammalian cancer spheroid derived from human mammary epithelial cells. This has been an important milestone for breast cancer research as the presence of potential breast
Cell therapy : Stem based cell therapy
Cell therapy refers to as the transfer of autologous or allogenic cellular material into a patient for medical purpose. In the year 1889, the first practices of cell therapy were done by Charles-Edouard Brown-Sequard in hormone therapy back then. He
BRAIN ORGANOIDS: NEURAL DEVELOPMENT AND NEUROLOGICAL DISEASES
A brain organoid is a self-organizing three-dimensional tissue structure derived from human embryonic stem cells or pluripotent stem cells. They show the ability to stimulate the architecture and functionality of the human brain. Recent publications have reported that brain organoids
Breakthroughs in glioblastoma research with neurosphere
Glioblastoma (GBM) is considered as among the most severe tumours across cancer types, and among brain tumours is considered most lethal. At present glioblastomas are incurable. These are aggressive tumours, infiltrating healthy neural tissues to the point of rendering surgical
Cultivating spheroids from primary cancers and cancer stem cells
Cancer stem cells constitute a fraction of cancer cells, that are able to self renew, and differentiate to generate entire tumor structures. These cells were discovered in the 1990s, in hemopoietic cancers, and subsequently reported to be present in solid
3D models of cancer cells
Tumor cells typically grow in 3-dimentional configurations. The surrounding tumor microenvironment is composed of non-tumor cells, and extracellular matrix (ECM). Within this microenvironment, tumor cells face hard growth conditions that include low oxygen exposure and nutrient levels and a susceptible
CELLULAR ASSAYS
Cellular assays or cell-based assays are essential tools for studying cellular mechanisms in live cells. Many cellular assays are designed and optimized for identifying or quantifying a specific response to an external stimulus. Assays can be divided into few categories
Stem cell spheroids – A vital tool in regenerative medicine
Stem cells serve as the primary resource in regenerative medicine including tissue engineering and transplantation therapy. To this effect human mesenchymal stem cells contain secretory factors with anti-inflammatory, angiogenic and immune regulation factors that makes them an efficient resource for
SCALE UP CULTURES AT INDUSTRIAL OR LARGE SCALE LEVELS
As the new technologies are evolving along with the new discoveries, there is a huge need to scale up and speed up the process of producing cells at a larger scale. Below are the commonly preferred devices used to scale