Cell lines are an easy and inexpensive tool used by researchers routinely. With the plethora of cell lines available today, selecting the right cell line for your project is fundamental for getting reliable results. Cell…
In the past decade research has experienced drastic changes in the development of an alternative research method over the traditional use of animal models as an investigative platform. 2D Monolayer in vitro cultures are effective…
Organoids are 3D complex cluster of cells, derived from either stem cells or organ specific progenitors. When these cells are provided with an external scaffold like Matrigel, they form a complex cluster of cells that…
Since the early 1900s, the two-dimensional (2D) cell culture method is used to culture cells and grow them in favorable artificial conditions (1) thereby playing a vital role in biomedical research (2). In line with…
Spheroids are three-dimensional structures that can be used as 3D cell culture models. It has been extensively shown that they mimic both architectural and structural property of human tissues. Spheroids play an increasingly crucial role…
The etymological origins of in vivo and in vitro comes from Latin, in vivo describes something within a living organism while in vitro describes something in glass such as a test tube or petri dish.…
Major requirement of a cell culture laboratory is the need to maintain an aseptic work area that is restricted to cell culture work. The simplest and most economical way to provide aseptic conditions is to…
Cells have been cultured since the 1940s and are generally used to investigate cell biology and molecular mechanism. Cells are taken directly from a tissue and after suitable preparation, transferred into an artificial environment. Cultures…
Animal models for specific diseases require a pre-existing insight into whether it’s the causative or a genetic condition. They are created by applying harmful conditions to animals or by manipulating the genes involved for a…
The wide ranges of in vitro culturing techniques presently employed are divided into two main categories namely, adherent cell culture and suspension culture. Adherent cell culture involves cultivating cells as monolayers on an artificial substrate.…