Three-dimensional cultures provide unique platform to study complex biological processes in vitro mainly cell proliferation, differentiation, motility, and stress. However, 3D spheroid culture methods are well established in certain tissue types, it is still challenging…
Spheroid culture system is a revolutionary 3D cell culture system that is relatively low tech, easy to use and easily reproducible. Spheroid system aids the formation of in-vivo like cell structure, where cells maintain their…
This article briefly describes the modern spheroid culturing techniques available. Pellet cultures In pellet culture systems, cells are forced to the bottom of the tube via centrifugation. Cell-cell adhesions are thus facilitated by proximity of…