Cancer disease being the second-leading cause of death worldwide is a highly studied subject (1). The models used by cancer researchers are continuously evolving and advancing to better understand cancer invasion, progression, and early detection.…
A three-dimensional (3D) cell culture is an artificially created environment in which cells grow or interact with their surroundings in all three dimensions. This cell culture is becoming increasingly popular to replace the traditional two-dimensional…
In the past decade research has experienced drastic changes in the development of an alternative research method over the traditional use of animal models as an investigative platform. 2D Monolayer in vitro cultures are effective…
Since the early 1900s, the two-dimensional (2D) cell culture method is used to culture cells and grow them in favorable artificial conditions (1) thereby playing a vital role in biomedical research (2). In line with…
Cell-based assays are one of the important pillars of the drug discovery process to provide a simple, fast, and cost-effective tool to avoid cost-intensive animal testing. To date, the traditional two-dimensional (2D) cell monolayers are…
Three-dimensional (3D) cell culture technology has become one of the main focuses of tumor cell biology, using its various methods and materials to mimic the in vivo microenvironment of tumor cells cultured ex vivo. 3D…
Two-dimensional (2D) cell cultures have been used by researchers since the early 1900s, but we are well aware of how growing cells on planar surfaces has some drawbacks. Cells grown in vitro in 2D space…
Pre-clinical cell-based assays have become a crucial tool in the advancement of biomedical and pharmaceutical studies, particularly in developing diagnostic tools, drug discovery, pathophysiological research, and regenerative medicine. Cell based assays allow for reduced use…
Spheroids are three-dimensional structures that can be used as 3D cell culture models. It has been extensively shown that they mimic both architectural and structural property of human tissues. Spheroids play an increasingly crucial role…
Animal models for specific diseases require a pre-existing insight into whether it’s the causative or a genetic condition. They are created by applying harmful conditions to animals or by manipulating the genes involved for a…