TRACING THE LINEGAE OF 3D CELL CULTURE
In late 19th century, it was demonstrated by Wilhelm Roux that it is possible to maintain living cells outside the body in saline buffer for a few days. (1). At the same time in parallel, Leo Loeb evaluated a technique
Cell culture media preparation: Significant impact on cell growth
The type of media used determines the success of mammalian cell culture. Most mammalian cells require specialized media supplemented with a complex mixture of nutrients, sugars, amino acids, vitamins, minerals, and growth factors such as insulin. The media provides energy
Mammalian Cell culture: The core tool in therapeutic protein production
Mammalian cell culture is a process of isolating cells from animal tissues and expanding them in-vitro in a dish or a flask. It is currently the foremost tool in basic physiology research, as well as in the production of therapeutic
Pluripotent cell culture techniques
Stem cells have the potential to differentiate into various types of cells. They function as a repair system for the body. There are two types of stem cells present in the body; embryonic stem cells and adult stem cells. They
Primary Hepatocyte Cell culture applicable for drug induced liver injury studies
Drug induced liver injury (DILI) is an adverse response to drugs or other xenobiotic chemicals. It can occur as a predictive event when a person is exposed to toxic amounts of some compounds or as an unpredictable event caused by