Stem cells serve as the primary resource in regenerative medicine including tissue engineering and transplantation therapy. To this effect human mesenchymal stem cells contain secretory factors with anti-inflammatory, angiogenic and immune regulation factors that makes them an efficient resource for…
Scaffold based cell culture systems namely organoids are reviewed as a promising option for studying biomedical applications. Human organoids are a tool for drug screening and study disease progression. These 3D cell cultures replicate important aspects of the in-vivo micro-environment…
Respiratory Syncytial Virus (RSV) is one of the most common respiratory tract viruses with high mortality rate among infants below the age of 5. Within the adult population it can cause chronic disease and weaker immunity. RSV is mainly found…
3D cultures are now being extensively used to study the effects of respiratory viruses on host tissues. 3D cultures contain a modifiable platform to culture cells, that allows for development of in-vivo like structure and function of cells, resulting in…
Traditionally cell based studies for respiratory viruses were performed on 2D monolayer cell cultures. In-vivo studies heavily relied on animal models. Both types of models however were not successful at accurately mimicking the in-vivo physiology and pathophysiology of cells in…
This article briefly describes the modern spheroid culturing techniques available. Pellet cultures In pellet culture systems, cells are forced to the bottom of the tube via centrifugation. Cell-cell adhesions are thus facilitated by proximity of single cells at the bottom.…
The type of media used determines the success of mammalian cell culture. Most mammalian cells require specialized media supplemented with a complex mixture of nutrients, sugars, amino acids, vitamins, minerals, and growth factors such as insulin. The media provides energy…
Mammalian cell culture is a process of isolating cells from animal tissues and expanding them in-vitro in a dish or a flask. It is currently the foremost tool in basic physiology research, as well as in the production of therapeutic…
Stem cells have the potential to differentiate into various types of cells. They function as a repair system for the body. There are two types of stem cells present in the body; embryonic stem cells and adult stem cells. They…
The brain is composed of a complex 3D interconnected system of different cells and extracellular matrixes (ECM). To mimic its 3D organization in-vivo, in-vitro culturing presents a unique challenge. The culture conditions should mimic the appropriate microenvironment with topographical cues…