Cells have been cultured since the 1940s and are generally used to investigate cell biology and molecular mechanism. Cells are taken directly from a tissue and after suitable preparation, transferred into an artificial environment. Cultures are kept in special dishes…
Organoids constitute an improved model system in which cell types more closely resemble the in vivo situation and are in similar conditions to those found in the human body. Diverse organoid systems provide a useful variety for their complexity and…
Organoid research has been an emerging filed in the past few decades since organoids have the potential to provide better cancer drug screening. While cell culture is important for determining whether a drug should advance to clinical trials, a study…
Induced pluripotent stem cells are a new type of pluripotent cells which can be obtained by reprogramming animal and human differentiated cells. Pluripotent stem cells are a unique model for studying a variety of processes that occur in the early…
The development of the organoid model was the result of decades of study in developmental biology, and organ physiology coupled with technological advancements in growing tissues ex vivo. With more and more focus being shifted to 3D cell culture from…
Organoids are self-organized 3D structures derived from adult or embryonic stem cells that are able to represent the structure and function of the derived tissue. They are able to accurately mimic tissue specific structure and function consistent with in-vivo tissue…
Solid tumors are composed of a number of different types of cells. These include cancer cells, stromal cells such as fibroblasts, immune cells, lymphatic endothelial cells, vascular endothelial cells, pericytes and adipocytes (1). These cells by it self are not…
3D cultures have become a vital tool in tumor related research and drug discovery. 3D cultures of tumor cell lines bear remarkable structural and physiological similarities to in vivo solid tumors. Spheroids of cancer cells are able to mimic the…
Cellular viability plays an important role when determining the efficacy and reliability of a MCTs model. There are many established viability assays such as colorimetric, luminescence and fluorescence assays. However these assays are well suited and optimized for traditional 2D…
Organoid technology is improving rapidly with effective incorporation of immune cells and vascularization, which often represents the major hurdles in this technology. Other advancements are to facilitate new discovery research and drug development efforts. Glioblastoma is known to invade along…