Cell culture or tissue culture is one of the techniques that are mostly used in life sciences in which cells are grown under controlled conditions, outside their natural environment. The cells or tissues, are removed from their original sources and…
Growth media remains a key component of 3D cell culture experiments ensuring cells can thrive outside their natural environment. The continuous development and optimization of cell culture media provide new insights into the association between cells and cell culture media…
Since the early 1900s, the two-dimensional (2D) cell culture method is used to culture cells and grow them in favorable artificial conditions (1) thereby playing a vital role in biomedical research (2). In line with the limitations of 2D cell…
Cell-based assays are one of the important pillars of the drug discovery process to provide a simple, fast, and cost-effective tool to avoid cost-intensive animal testing. To date, the traditional two-dimensional (2D) cell monolayers are the preferred method to culture…
Three-dimensional (3D) cell culture technology has become one of the main focuses of tumor cell biology, using its various methods and materials to mimic the in vivo microenvironment of tumor cells cultured ex vivo. 3D culture techniques promise to be…
Two-dimensional (2D) cell cultures have been used by researchers since the early 1900s, but we are well aware of how growing cells on planar surfaces has some drawbacks. Cells grown in vitro in 2D space don’t behave like cells found…
Pre-clinical cell-based assays have become a crucial tool in the advancement of biomedical and pharmaceutical studies, particularly in developing diagnostic tools, drug discovery, pathophysiological research, and regenerative medicine. Cell based assays allow for reduced use of expensive animal models and…
Stem cells have the capacity to self-renew to produce additional stem cells by process of mitosis. They can be classified as multipotent (ability to give rise to multiple cells within a lineage), pluripotent (ability to give rise to cell types…
Spheroids are three-dimensional structures that can be used as 3D cell culture models. It has been extensively shown that they mimic both architectural and structural property of human tissues. Spheroids play an increasingly crucial role in the context of drug…
Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the inner cell mass of human embryos in the blastocysts stage of development. They show an unlimited capacity to self-renew in culture systems both in vivo and in vitro. …