This article briefly describes the modern spheroid culturing techniques available. Pellet cultures In pellet culture systems, cells are forced to the bottom of the tube via centrifugation. Cell-cell adhesions are thus facilitated by proximity of…
The type of media used determines the success of mammalian cell culture. Most mammalian cells require specialized media supplemented with a complex mixture of nutrients, sugars, amino acids, vitamins, minerals, and growth factors such as…
Mammalian cell culture is a process of isolating cells from animal tissues and expanding them in-vitro in a dish or a flask. It is currently the foremost tool in basic physiology research, as well as…
Stem cells have the potential to differentiate into various types of cells. They function as a repair system for the body. There are two types of stem cells present in the body; embryonic stem cells…
Primary cell cultures are ex-vivo cultures produced from cells that are freshly harvested from a multicellular organism. Generally, primary cell cultures are more representative of in-vivo tissues than immortalized cell lines (1). However culturing primary…
The terms spheroids and organoids are often used interchangeably. They are similar in many ways but also different in what they are made up of, and what they do. Depending on the type of data…
Cancer research relies on cell based model systems to understand the growth and progression of tumors, and subsequently the discovery of new therapeutic drugs. Traditional monolayer cultures have been widely used in this regard due…
The brain is composed of a complex 3D interconnected system of different cells and extracellular matrixes (ECM). To mimic its 3D organization in-vivo, in-vitro culturing presents a unique challenge. The culture conditions should mimic the…
Induced pluripotent stem cells (iPSCs) are a pluripotent stem cell line that is produced directly from a somatic cell. The technology is based on the principle that somatic cells can be converted in to pluripotent…
Biofloat coated plates are easy to use, versatile and reliable. There is no specific protocol for using the plates, however, for maximum reliability and reproducibility of experiments, please follow the following guidelines. 1. Seeding density-…