Mammalian cell culture is a process of isolating cells from animal tissues and expanding them in-vitro in a dish or a flask. It is currently the foremost tool in basic physiology research, as well as in the production of therapeutic…
Stem cells have the potential to differentiate into various types of cells. They function as a repair system for the body. There are two types of stem cells present in the body; embryonic stem cells and adult stem cells. They…
Primary cell cultures are ex-vivo cultures produced from cells that are freshly harvested from a multicellular organism. Generally, primary cell cultures are more representative of in-vivo tissues than immortalized cell lines (1). However culturing primary cells are not without its…
The terms spheroids and organoids are often used interchangeably. They are similar in many ways but also different in what they are made up of, and what they do. Depending on the type of data required from 3D culture, it…
Cancer research relies on cell based model systems to understand the growth and progression of tumors, and subsequently the discovery of new therapeutic drugs. Traditional monolayer cultures have been widely used in this regard due to its ability to generate…
The brain is composed of a complex 3D interconnected system of different cells and extracellular matrixes (ECM). To mimic its 3D organization in-vivo, in-vitro culturing presents a unique challenge. The culture conditions should mimic the appropriate microenvironment with topographical cues…
Induced pluripotent stem cells (iPSCs) are a pluripotent stem cell line that is produced directly from a somatic cell. The technology is based on the principle that somatic cells can be converted in to pluripotent stem ells, by the introduction…
Biofloat coated plates are easy to use, versatile and reliable. There is no specific protocol for using the plates, however, for maximum reliability and reproducibility of experiments, please follow the following guidelines. 1. Seeding density- The seeding density is dependent…
Drug induced liver injury (DILI) is an adverse response to drugs or other xenobiotic chemicals. It can occur as a predictive event when a person is exposed to toxic amounts of some compounds or as an unpredictable event caused by…
Three-dimensional (3D) cultures are widely accepted as an excellent in-vitro model of high through out drug screening, preclinical cancer research and cancer/stem cell research. 3D spheroid models are morphologically and functionally representative of in-vivo cell state, and are able to…