Respiratory Syncytial Virus (RSV) is one of the most common respiratory tract viruses with high mortality rate among infants below the age of 5. Within the adult population it can cause chronic disease and weaker immunity. RSV is mainly found…
3D cultures are now being extensively used to study the effects of respiratory viruses on host tissues. 3D cultures contain a modifiable platform to culture cells, that allows for development of in-vivo like structure and function of cells, resulting in…
Traditionally cell based studies for respiratory viruses were performed on 2D monolayer cell cultures. In-vivo studies heavily relied on animal models. Both types of models however were not successful at accurately mimicking the in-vivo physiology and pathophysiology of cells in…
In the past 2 years, with the Covid-19 pandemic we have witnessed how a single virus can cripple the economy of nations and impact every day life. Respiratory viral infections, which includes severe acute respiratory syndrome coronaviruses (SARS-CoV) remains among…
High throughput screening (HTS) is a technique utilised in scientific experimentation in fields of biology, materials science and chemistry. It has become the method of choice in drug discovery processes. HTS utilises advanced robotics, data processing software, liquid handling devices…
Scaffold free 3D cultures are cultivated in specialised cell repellant culture plates that causes cells to self aggregate. These involve hanging drop microplates, low adhesion plates with specialised polymer coating such as BIOFLOAT 96-well plates that encourages spheroid formation, or…
Scaffold dependent 3D cell cultures rely on physical support that ranges from mechanical surfaces to extra cellular matrix (ECM) like structures on which cells are cultivated to aggregate, divide and migrate. The physical and chemical properties of the scaffold will…
Spheroid culture system is a revolutionary 3D cell culture system that is relatively low tech, easy to use and easily reproducible. Spheroid system aids the formation of in-vivo like cell structure, where cells maintain their native phenotype, and extra-cellular matrix…
This article briefly describes the modern spheroid culturing techniques available. Pellet cultures In pellet culture systems, cells are forced to the bottom of the tube via centrifugation. Cell-cell adhesions are thus facilitated by proximity of single cells at the bottom.…
The type of media used determines the success of mammalian cell culture. Most mammalian cells require specialized media supplemented with a complex mixture of nutrients, sugars, amino acids, vitamins, minerals, and growth factors such as insulin. The media provides energy…