A human model system is preferable to animal models when studying infectious diseases pathogenesis because pathogens often have a narrow species or tissue tropism, meaning that they infect only certain species and sometimes certain cell types. For decades there were…
Animal models for specific diseases require a pre-existing insight into whether it’s the causative or a genetic condition. They are created by applying harmful conditions to animals or by manipulating the genes involved for a particular disorder respectively, whereas three…
Human organoid system fundamentally mimic a part of the human body but not the entire body. Organoid system lacks the interorgan communication. Therefore, human organoids are limited to reproduction of organ-specific or tissue-specific micro physiology, a limitation to bear in…
Spheroid models used in drug screens offer the benefit of a physiologically relevant biological model to allow for screening of cancer killing therapeutics or drugs toxicity to host cells. Spheroid culture systems are easy to use, modifiable, adaptable to standards…
The wide ranges of in vitro culturing techniques presently employed are divided into two main categories namely, adherent cell culture and suspension culture. Adherent cell culture involves cultivating cells as monolayers on an artificial substrate. Suspension culture involves culturing cells…
A brain organoid is a self-organizing three-dimensional tissue structure derived from human embryonic stem cells or pluripotent stem cells. They show the ability to stimulate the architecture and functionality of the human brain. Recent publications have reported that brain organoids…
Hubert et al. were the first to report culturing glioblastoma as a more complex organoid composed of multiple cell types rather than relatively homogenous 3D spheroid. They had successfully created these GBM organoids from patient derived primary cultures, xenografts and…
Animal models for specific diseases require a pre-existing insight into whether it’s the causative or a genetic condition. They are created by applying harmful conditions to animals or by manipulating the genes involved for a particular disorder respectively, whereas three…
Human organoids are a vital source for precision medicine. They can be useful to select an appropriate drug for the patients with genetic diseases or cancer. Cystic fibrosis is a relatively common genetic disease with approximately 90,000 patients suffering with…
The advances in spheroid technology in particular, neurospheres has led to establishment of “mammospheres”; a mammalian cancer spheroid derived from human mammary epithelial cells. This has been an important milestone for breast cancer research as the presence of potential breast…