Solid tumors are composed of a number of different types of cells. These include cancer cells, stromal cells such as fibroblasts, immune cells, lymphatic endothelial cells, vascular endothelial cells, pericytes and adipocytes (1). These cells by it self are not…
3D cultures have become a vital tool in tumor related research and drug discovery. 3D cultures of tumor cell lines bear remarkable structural and physiological similarities to in vivo solid tumors. Spheroids of cancer cells are able to mimic the…
Cellular viability plays an important role when determining the efficacy and reliability of a MCTs model. There are many established viability assays such as colorimetric, luminescence and fluorescence assays. However these assays are well suited and optimized for traditional 2D…
Organoid technology is improving rapidly with effective incorporation of immune cells and vascularization, which often represents the major hurdles in this technology. Other advancements are to facilitate new discovery research and drug development efforts. Glioblastoma is known to invade along…
Spheroids are recognized as physiologically relevant three-dimensional models that could capture the major characteristics of both healthy and diseased tissues. Considering its high cell density, increased deposition of extracellular matrix and accelerated proliferation rates, spheroid-based tumour models can greatly reduce…
Despite the much advancement seen in tumor related research and drug discovery, cancer research still experiences a significant amount of challenges due the complex behaviour of tumor cells. Multicellular tumor spheroids (MCTs) bear remarkable similarities to in vivo solid tumors…
Spheroids, which are self, assembled cellular aggregates, as a representative 3D model of solid tumors is largely dependent on the uniformity of its size and shape. Uniform spheroids yield reproducible results in drug screens as well as obtaining meaningful findings…
Multicellular tumor (MCT) spheroids are an essential tool in tumor related research, and drug screens. MCT can be defined as aggregates of heterogeneous cells, closely packed with highly dense spheroids. In order to use MCTs in physiological research and clinical…
Spheroid model has been shown to have features consistent with patient specific Non-Small Cell Lung Cancer Spheroid tumours (NSCLC), characterised by extremely high clonal and morphological heterogeneity of tumours and multi-drug resistance. Lung malignancies accounts for 11% of cancer worldwide…
Multicellular tumor (MCT) spheroids are an essential tool in tumor related research, and drug screens. MCT can be defined as aggregates of heterogeneous cells, closely packed with highly dense spheroids. These cells exhibit strong cell-cell and cell-extracellular matrix (ECM) interactions…