The etymological origins of in vivo and in vitro comes from Latin, in vivo describes something within a living organism while in vitro describes something in glass such as a test tube or petri dish. An in vivo study involves…
Major requirement of a cell culture laboratory is the need to maintain an aseptic work area that is restricted to cell culture work. The simplest and most economical way to provide aseptic conditions is to use a cell culture hood.…
Using organoids to study immunotherapy has been an emerging technique. In a study published in 2019, a method of co-culturing tumour organoids with peripheral blood lymphocytes generated immune cells that recognize and kill autologous tumour organoids. This method preserves the…
A spheroid culture system provides a similar physiochemical environment to in vivo, by facilitating cell-cell and cell-matrix interaction to overcome the limitations of traditional monolayer cell cultures. Due to the importance of stem cell culture systems in clinical applications, researchers…
Cells have been cultured since the 1940s and are generally used to investigate cell biology and molecular mechanism. Cells are taken directly from a tissue and after suitable preparation, transferred into an artificial environment. Cultures are kept in special dishes…
Organoids constitute an improved model system in which cell types more closely resemble the in vivo situation and are in similar conditions to those found in the human body. Diverse organoid systems provide a useful variety for their complexity and…
Organoid research has been an emerging filed in the past few decades since organoids have the potential to provide better cancer drug screening. While cell culture is important for determining whether a drug should advance to clinical trials, a study…
Induced pluripotent stem cells are a new type of pluripotent cells which can be obtained by reprogramming animal and human differentiated cells. Pluripotent stem cells are a unique model for studying a variety of processes that occur in the early…
The development of the organoid model was the result of decades of study in developmental biology, and organ physiology coupled with technological advancements in growing tissues ex vivo. With more and more focus being shifted to 3D cell culture from…
Organoids are self-organized 3D structures derived from adult or embryonic stem cells that are able to represent the structure and function of the derived tissue. They are able to accurately mimic tissue specific structure and function consistent with in-vivo tissue…